تغییرات محتوای سولفورافان و فعالیت برخی آنزیم‌های آنتی‌اکسیدان گیاهچه‌های ازمک (Lepidium draba L.) در پاسخ به جاسمونات

Sulforaphane is an isothiocyanate which derived of glucoraphanin (a kind of glucosinolate) under hydrolysis of myrosinase enzyme, has shown various pharmaceutical properties such as anticancer activity in human. This glucosinolate abundantly found in Lepidium draba L. of Brassicaceae family. The goal of this research was investigation of the variation in sulforaphane, total flavonoid and anthocyanin contents and also investigation of the activity of catalase and peroxidase enzymes in 5-day-old L. draba seedlings in treatment of various concentration of jasmonate. The obtained results showed whereas the sulforaphane content was not changed after 48 hours treatment, its content significantly decreased in 50 and 100 μM jasmonate treatment after 72 hours. On the other hand, total flavonoid content were increased in the 50 and 100 μM jasmonate treated seedlings at the both of treatment times and the anthocyanin content was also significantly evaluated in the 72 hours-treated seedlings in compared to the control. Furthermore, activity of catalase and peroxidase enzymes reduced at both of treatment times (except peroxidase activity in treated with 50 μM jasmonat after 72 hours). Overall, it deduced that jasmonate effect depends on its concentration, time of treatment as well as metabolite biosynthesis pathway. Furthermore, it seems under jasmonat treatment, the antioxidant system of this plant improved more through phenylpropanoid pathway.